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KMID : 0364820150510010014
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Korean Journal of Microbiology
2015 Volume.51 No. 1 p.14 ~ p.20
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Plant growth-promoting activity and identification of endophytic fungi isolated from native plant in East coast
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Yoo Young-Hyun
Jin Yong-Joo
Kang Sang-Mo
Oh Se-Jong
Lee Myung-Chul
Kim Jong-Kuk
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Abstract
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Coastal plant species, Plantago camtschatica Cham. native to the coastal region of the East Sea were sampled and then morphologically different 20 endophytic fungal strains were purely isolated. Phylogenetic analysis of isolates was done by the Bayesian program based on sequenced internal transcribed spacer (ITS-rDNA) region. Culture filtrates of each of 20 isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity, respectively. As the results, E/PC/10/1 strain showed the highest plant growth-promoting activity among them. The culture filtrate of the strain E/PC/10/1 was revealed as containing gibberellins (GA1, GA3, GA4) by using HPLC, and gas GC/MS with selected ion monitoring (SIM). Finally, this strain was identified as novel Penicillium spinulosum species that producing new GAs with microscopic observation and further molecular analysis with beta-tubulin gene sequence.
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KEYWORD
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Penicillium spinulosum, endophytic fungi, gibberellin, plant growth-promoting activity
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